Flowjo compensation manual

FlowJo computes the compensation matrix on control samples much the way you would manually set the compensation. To do this, you will select gates on positive and negative populations for each of these stain, and tell FlowJo to calculate the compensation matrix based on these stains. Manual versus Software Compensation. Manual creation of the compensation matrix is possible but requires a large amount of work. Because of the exponential growth in the matrix size, it is not feasible with more than about parameters. As an exercise, lets walk through how to manually compensate using a two color FITC and PE example. Welcome to FlowJo, a software application with an integrated environment for viewing and analyzing flow cytometric data.. The environment is presented as the Workspace, which contains a list of loaded samples (experimental data), statistics, gates, and other analyses, as well as tabular and graphical www.doorway.ru workspace is saved as a FlowJo document on your hard .

FlowJo Flojo help manual guide lesson help. Text in the Wizard will guide you through the process until you have the right setup to create a compensation matrix. Compensation Example. This is a simple example showing how to do three-color compensation in FlowJo. There are several steps involved. Set a gate for the positive and negative populations for each singly-stained sample. Bring up the Compensation Definition dialog window, or Compensation Wizard. Drag each of the positive and negative populations. The Compensation Wizard in FlowJo is one of the most frequently used platforms, and by extension potentially the greatest source of confusion on a per-cytometrist basis. This brief guide is to help users new to compensation calculations and experienced flow-maestros alike breeze through this process in a painless and professional.

Starting with FlowJo version , you can easily change the compensation matrix values. When you create a new compensation matrix, FlowJo no longer automatically saves it to a file. You can edit the compensation matrices directly from FlowJo, using the Platform- Compensate Sample- Edit/Save Matrix command (from the Platform - Compensate Sample menu, you can also Remove Compensation). The workspace is saved as a FlowJo document on your hard disk; when you reopen the document, you will see your analysis as it was when it was last saved. Our web manual is a search-able set of pages which explains the many features and functions in FlowJo. Compensation. Compensation has undergone a major redesign for FlowJo Version 10! Compensation in flow cytometry is the process of correcting for fluorescence spillover emissions. The detectors, or channels, in the instrument are designed to detect a very specific range of emissions. However, the fluorophores used in flow cytometry do not adhere.